Journal: Drug Delivery

Article Title: Improved oral absorption and anti-lung cancer activity of paclitaxel-loaded mixed micelles

doi: 10.1080/10717544.2016.1245370

Figure Lengend Snippet: Tumor volumes of C57BL/6 mice implanted with Lewis cells in control, PTX, PTX-TP-M groups (panel A). The results are presented as the mean ± SD ( n = 6); pathological section images of the tumor tissues of control and Lewis tumor-bearing C57BL/6 mice treated by PTX and PTX-TP-M (panel B). Scale bar = 20 or 50 μm.

Article Snippet: A549 and Lewis lung cancer cells and basal Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Nanjing KeyGEN Biotech.

Techniques: Control

Journal: International Immunology

Article Title: Cancer-derived cholesterol sulfate is a key mediator to prevent tumor infiltration by effector T cells

doi: 10.1093/intimm/dxac002

Figure Lengend Snippet: SULT2B1b-mediated CS production in tumors dampens anti-tumor T-cell responses. (A) The effect of OTI CD8 + T-cell transfer on tumor growth in C57BL/6 mice was compared between E.G7-OVA-MOCK ( n = 5) and E.G7-OVA-SULT ( n = 6). After the intravenous injection of activated Vα2 + Vβ5 + OTI T cells on day 10, the infiltration of OTI T cells was analyzed on day 15 ( n = 7). Data are presented as the mean + SD or ± SD. * P < 0.05, ** P < 0.01 (two-tailed Mann–Whitney test for both tumor growth and infiltration of OTI T cells). (B) The effect of OTI CD8 + T-cell transfer on tumor growth in C57BL/6 mice was compared between E0771-OVA-control ( n = 8) and E0771-OVA-SULT ( n = 8). After the intravenous injection of activated Vα2 + Vβ5 + OTI T cells on day 10, the infiltration of OTI T cells was analyzed on day 15 ( n = 7 or 8). Data are presented as the mean + SD or ± SD. * P < 0.05, ** P < 0.01 (two-tailed Mann–Whitney test for tumor growth and two-tailed unpaired Student’s t- test for infiltration of OTI T cells). (C) Schematic representation of the interaction between OTII CD4 + T cells and tumor cells expressing I-A b molecule covalently bound to OVA peptide. (D and E) The effect of OTII CD4 + T-cell transfer on tumor growth in C57BL/6 mice was compared between (D) E0771-control-I-A b /OVA ( n = 8) and E0771-SULT-I-A b /OVA ( n = 7) or (E) 3LL-control-I-A b /OVA ( n = 8) and 3LL-SULT-I-A b /OVA ( n = 8). After the intravenous injection of activated Vα2 + Vβ5 + OTII T cells on day 9, infiltration of OTII T cells was analyzed on day 14 (D, n = 9; E, n = 7). Data are presented as the mean + SD or ± SD. * P < 0.05, ** P < 0.01 (two-tailed unpaired Student’s t- test for tumor growth and two-tailed Mann–Whitney test for infiltration of OTII T cells). (F) Comparison of the effect of DOCK2 +/+ OTII CD4 + T cells and DOCK2 –/– OTII CD4 + T cells ( n = 7) on the growth of E0771-control-I-A b /OVA transplanted into C57BL/6 mice. Data are presented as the mean + SD. * P < 0.05 (two-tailed unpaired Student’s t- test).

Article Snippet: 3LL cells were obtained from Japanese Collection of Research Bioresources (Osaka, Japan), and MC38 cells were a gift from Dr S. A. Rosenberg (National Cancer Institute, Bethesda, MD, USA) and were previously reported elsewhere ( ).

Techniques: Injection, Two Tailed Test, MANN-WHITNEY, Control, Expressing, Comparison

Journal: British Journal of Cancer

Article Title: A novel hypoxia-dependent 2-nitroimidazole KIN-841 inhibits tumour-specific angiogenesis by blocking production of angiogenic factors

doi: 10.1038/sj.bjc.6600667

Figure Lengend Snippet: Inhibitory effect of KIN-841 on proliferation of BPAE cells and 3LL cells under normoxia. Cells were treated for 48 h with KIN-841 at the various concentrations indicated. a P <0.05 vs controls; b P <0.05 vs normoxia.

Article Snippet: Lewis lung carcinoma and cultured Lewis lung carcinoma (3LL) cells were from Dr T Sakurai (Keio University, Tokyo, Japan).

Techniques:

Journal: British Journal of Cancer

Article Title: A novel hypoxia-dependent 2-nitroimidazole KIN-841 inhibits tumour-specific angiogenesis by blocking production of angiogenic factors

doi: 10.1038/sj.bjc.6600667

Figure Lengend Snippet: Inhibitory effect of KIN-841 on proliferation of 3LL cells under hypoxia. Cells were treated for 48 h with KIN-841 at the various concentrations indicated under normoxia or hypoxia. a P <0.05 vs controls; b P <0.01 vs controls; c P <0.05 vs normoxia.

Article Snippet: Lewis lung carcinoma and cultured Lewis lung carcinoma (3LL) cells were from Dr T Sakurai (Keio University, Tokyo, Japan).

Techniques:

Journal: British Journal of Cancer

Article Title: A novel hypoxia-dependent 2-nitroimidazole KIN-841 inhibits tumour-specific angiogenesis by blocking production of angiogenic factors

doi: 10.1038/sj.bjc.6600667

Figure Lengend Snippet: Inhibitory effect of KIN-841 on the production of angiogenic factor in 3LL cells under hypoxia. ( A ) Effect of 3LL cell-CM treated with KIN-841 at the various concentrations indicated under normoxia or hypoxia on [ 3 H]thymidine incorporation in BPAE cells. a P <0.01 vs normoxia controls; b P <0.01 vs hypoxia controls. ( B ) Effects of KIN-841 and anti-VEGF antibody on stimulation of [ 3 H]thymidine incorporation in BPAE cells by 3LL cell-CM incubated under normoxia or hypoxia. a P <0.05 vs normoxia controls; b P <0.05 vs normoxia controls; c P <0.01 vs hypoxia controls. ( C ) VEGF concentration in 3LL cell-CM treated with KIN-841 at the various concentrations indicated under normoxia or hypoxia. a P <0.05 vs normoxia controls; b P <0.05 vs hypoxia controls; c P <0.01 vs hypoxia controls.

Article Snippet: Lewis lung carcinoma and cultured Lewis lung carcinoma (3LL) cells were from Dr T Sakurai (Keio University, Tokyo, Japan).

Techniques: Incubation, Concentration Assay

Journal: British Journal of Cancer

Article Title: A novel hypoxia-dependent 2-nitroimidazole KIN-841 inhibits tumour-specific angiogenesis by blocking production of angiogenic factors

doi: 10.1038/sj.bjc.6600667

Figure Lengend Snippet: Inhibition of 3LL-cell-induced angiogenesis by KIN-841 as determined using the mouse dorsal air sac method. Dorsal skin of the mice 7 days after implantation of a chamber filled with PBS alone ( A ) or 3LL cells ( B and C ). The implanted mice were treated with 200 mg kg −1 day −1 of KIN-841 for 4 days between days 1 and 6, intraperitoneally ( C ). In ( D ), the grade of angiogenesis induced by 3LL cells is shown using the indexes described in Materials and Methods. a P <0.01 tumour-free controls; b P <0.05 vs tumour controls.

Article Snippet: Lewis lung carcinoma and cultured Lewis lung carcinoma (3LL) cells were from Dr T Sakurai (Keio University, Tokyo, Japan).

Techniques: Inhibition